These are the services provided by Translational Proteomics Center (TPC):

• Experimental design and power analyses*
• Depletion of most abundant proteins
• BCA (Total Protein Quantitation)
• Immunoprecipitation
  • >> Investigators must provide the antibodies and the lysis buffer.
• Sample Preparation:
  • Acetone Precipitation
  • SDS PAGE
  • Coomassie or Silver Stain
• Sample Processing for Mass Spectrometry:
  • Reduction and Alkylation
  • Enzymatic digestion in-gel
  • Peptide Extractions
  • Peptide cleaning (when required)
• Protein Identification:
  • Analysis by LC-MS/MS (Easy nLC 1000/LTQ XL)
  • Proteome Discoverer 1.4 or 2.1 Data Search
  • Proteome Discoverer 2.5
• Quantitative Proteomics:
  • Peptide tagging (TMT labeling)
  • Analysis by LC-MS/MS (Easy nLC 1200/Q-Exactive Plus)
  • Peptide Fractionation
  • Proteome Discoverer 2.1 Data Search
• Targeted mass spectrometry analysis
  • Processed samples are run in the Q-Exactive Plus
  • Peptide sequences and charge states must be provided using previous (non-targeted) mass spectrometry data
  • Raw data analysis not included

Notes:

• An initial consultation with Dr. Loyda M. Meléndez and Staff is required to determine the experimental approach. 
• Quantitative Proteomics by TMT Labelling requires a supply kit ($3,000 x 30 samples) included in the final Invoice.
• Requires Bio-informatics analysis. Data generated by Mass Spectrometry will be transferred directly to Dr. Abiel Roche’s facility. Additional costs apply that should be coordinated with Dr. Roche.
• (*) Services that can also be provided in collaboration with Dr. Horacio Serrano’s QP Lab.