TPC Services
- Experimental design and power analyses*
- Depletion of most abundant proteins
- BCA (Total Protein Quantitation)
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Immunoprecipitation
- >> Investigators must provide the antibodies and the lysis buffer.
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Sample Preparation:
- Acetone Precipitation
- SDS PAGE
- Coomassie or Silver Stain
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Sample Processing for Mass Spectrometry:
- Reduction and Alkylation
- Enzymatic digestion in-gel
- Peptide Extractions
- Peptide cleaning (when required)
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Protein Identification:
- Analysis by LC-MS/MS (Easy nLC 1000/LTQ XL)
- Proteome Discoverer 1.4 or 2.1 Data Search
- Proteome Discoverer 2.5
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Quantitative Proteomics:
- Peptide tagging (TMT labeling)
- Analysis by LC-MS/MS (Easy nLC 1200/Q-Exactive Plus)
- Peptide Fractionation
- Proteome Discoverer 2.1 Data Search
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Targeted mass spectrometry analysis
- Processed samples are run in the Q-Exactive Plus
- Peptide sequences and charge states must be provided using previous (non-targeted) mass spectrometry data
- Raw data analysis not included
Notes:
- An initial consultation with Dr. Loyda M. Meléndez and Staff is required to determine the experimental approach.
- Quantitative Proteomics by TMT Labelling requires a supply kit ($3,000 x 30 samples) included in the final Invoice.
- Requires Bio-informatics analysis. Data generated by Mass Spectrometry will be transferred directly to Dr. Abiel Roche’s facility. Additional costs apply that should be coordinated with Dr. Roche.
- (*) Services that can also be provided in collaboration with Dr. Horacio Serrano’s QP Lab.