Neurogenetics & Bio-imaging Services
Since 2011, the Translational Neurogenetics Program (TNP) core facility at the MSC’s Institute of Neurobiology has provided the UPR biomedical research community with cutting edge neurogenetics support, cell culture facilities, imaging and image analysis instrumentation, training, and technical support. The TNP will transition to become the Neurogenetics and Bioimaging Service (NBS) of the RCMI Research Infrastructure Core. Current Director, Dr. Mark Miller, will become the Service Lead.
The NBS acts as a shared resource to assist investigators conducting studies addressing genetic mechanisms in neurobiological systems. The unit provides advisory, technical and instrumentation support. Major instrumentation includes: two temperature and humidity controlled incubators, two genetics workstations (“fly pushing”), a facility for preparation of fly food, and a stock of more than 1600 mutants and transgenic Drosophila lines. This core also contains all equipment and reagents needed to carry out basic molecular experiments. The Molecular Neurogenetics Research Unit is maintained on a day-to-day basis by Mr. Luis Quiñones who provides support to all users.
Bioimaging. A Nikon A1R Laser Scanning Confocal Microscope (LSCM) was purchased in 2013 with funding from the Major Research Instrumentation (MRI) program of the National Science Foundation. A Zeiss Pascal Laser Scanning Confocal Microscope, purchased in 2004, is maintained as a backup for the A1R, and is presently used mainly for training and analytical applications. An independent LSCM confocal system is available for applications that require imaging of fluorescent signals in living cells. The LSCM produced by Intelligent Imaging Innovations (3i), was purchased in 2011 with support from the Department of Defense. It uses spinning disk technology to gather data rapidly with minimal photo-bleaching. Unlike traditional instruments, which scan one point of laser light across an entire field, the spinning-disk confocal scans approximately 1,000 points of laser light across the field simultaneously, resulting in much faster image production and minimal specimen damage. Image analysis is performed on a free-standing computer system with the Imaris software module, which enables visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets.
On October 12, 2014, the University of Puerto Rico signed a Memorandum of Understanding establishing the UPR as one of fourteen Nikon Centers of Excellence (CoE) in North America. This university-industry partnership enables our RCMI Neuroimaging Core to purchase high-end research microscopes at favorable rates and facilitates access to Nikon technical knowledge and support.
Neuroscientist-in-Residence program. Dr. Daniel Colón Ramos, Yale Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale School of Medicine, has served as RCMI Neuroscientist-in-Residence since 2011 Dr. Colón Ramos presents seminars, meets with faculty and students, and collaborates on the National Science Foundation soliciting continued funding for the Center for Research Excellence in Science and Technology (CREST) Puerto Rico Center for Environmental Neuroscience.
Human Genetics Lab