Tropical and Infectious Diseases Research Services

The Tropical and Emerging Infectious Diseases Research Services (TEIDRS) provides instrumentation, facilities and key services to support and leverage research projects on Infectious Diseases disproportionally affecting Puerto Rico, the United States, and the world. These services build upon resources developed by the RCMI Infectious and Global Diseases Research Program (IGDRP). The facility is equipped with a suite of instrumentation, including a SpectraMax M3 multi-mode reader for UV-visible absorbance, fluorescence intensity, and glow luminescence measurements on microplates and cuvettes; an AMAXA Nucleofector II electroporator used to transfect human cells, yeasts, parasites, and hard-to-transfect cell lines, as well as bacteria; a CHEF-DR III electrophoresis apparatus with a cooling system to separate large DNA molecules such as malarial chromosomes (karyotypes), for multidrug resistant bacterial fingerprinting; a Nikon Eclipse E800 fluorescence microscope with digital camera including image analysis software; Gel Doc apparatus with Quantity One software; Veriti 96-well thermocycler, bench-top centrifuge 5810R from Eppendorf; SmartSpec Plus Spectrophotometer; Versafluor fluorometer; BioRad iCycler thermocycler; microplate reader with Microplate Manager software and filters for protein and nucleic acid determinations. There is also a dedicated tissue culture, biosafety level II containment facility with glass sliding door, equipped with a biosafety cabinet (Type II), water bath, bench top autoclave, refrigerated incubator, and automated cell counter. 

 

The TEIDRS is supervised by Adelfa Serrano, PhD, Professor of Microbiology, who serves as the Lead of the unit. She is assisted by Emilee E. Colón-Lorenzo, PhD, who is responsible for daily operations. Dr. Colon-Lorenzo has knowledge and expertise in specialized assays, including nucleofection transfection, drug sensitivity assays in vivo and in vitro, and in silico drug screening. Specialized services, trainings, and consultation on experimental design and instrument capabilities are provided to the users. Sophisticated equipment is operated by dedicated, appropriately trained staff.

Current ongoing services and support includes a total of 68 research projects such as: Amplification of Veillonella parvula in dental plaque (R01DE022559); Identification of biomarkers of chronic arthralgia after Chikungunya infection (U54MD007587-NIMHD); Vaccine development against infectious diseases (1SC3GM116698); Novel inhibitors of the malarial GST protein - from bench to a marketable drug (competitive grant from Puerto Rico Science, Technology & Research Trust), and Indoor Molds at the Caño Martin Peña (EPA Contract). Specialized IGDRP resources are also used for research projects in other areas, such as; Mitochondrial permeability transition as a target for cardio-protection in heart failure (SC1HL118669); Mitochondrial DNA and APE1 in Huntington’s Disease (SC1GM113757); Development and validation of a nonhuman primate model of pre-diabetes and pharmacological interventions (P40OD012217); KM Zeta involvement in cocaine sensitization (SC1GM084854); Biophysical properties of dopaminergic neurons (NSF PIRE OISE-1545803); Nanoparticles toxicology (NSF CREST 1345156), and Endocytic mechanisms controlling functional selectivity of the CB1R (RO1DA037924).

 

 

 

Emilee Colón-Lorenzo, PhD.

Adjunct Professor
E-mail: emilee.colon@upr.edu