The mission of the RCMI Translational Proteomics Center (TPC) is to enhance the current capacity for biomedical research at the UPR-MSC. Loyda M. Meléndez, PhD, currently serves as Director of the Proteomics Service (PS) and Lead of the Research Infrastructure Core.
The Proteomics Service functions as a platform for biomarker discovery, protein identification and quantitation, and as an interface between basic and clinical research conducted in Puerto Rico for the diagnosis and therapeutic monitoring of human diseases. This core facility provides resources and expertise in Proteomics to scientists in the all the RCMI programs in Puerto Rico (UPR-Medical Sciences Campus, Ponce School of Medicine, and Universidad Central del Caribe), partner institutions in the PRINBRE Program, and other campuses of the UPR System. The facility is well equipped with a LTQ XL and a Q Exactive™ Hybrid Quadrupole Orbitrap Mass Spectrometer from Thermo Scientific™. This is the backbone of the proteomics platform for the rapid discovery of multiple biomarkers, for protein identification and protein quantitation. Current studies supported by the TPC include but are not limited to:
Targeting monocyte/macrophage Cathepsin B in HIV-1 neurocognitive disorders (5SC1GM113691);
The novel RAC inhibitors as potential therapeutics for metastatic breast cancer (PRINBRE CNIH/NIGMS P20GM103475);
Exosome-mediated soluble insulin receptor secretion correlates with HAND (R01NS099036).
The TPC and GTRU share a license for the Ingenuity Pathway Analysis platform for network, canonical pathways, and other types of system biology analyses, and to assist in the identification of new targets or candidate biomarkers.
- Provide an integrated platform for biomarker discovery and protein identification and quantitation to interface between basic and clinical research conducted in Puerto Rico for the diagnosis and therapeutic monitoring of human diseases.
- Integrate Genomics, Bioinformatics (Ingenuity Pathways), Metabolomics (INBRE), and Emergent Infectious Disease Cores with Proteomics Platform.
- Provide TPC services with new methodologies on quantitative proteomics such as: TMT labelling.
- Promote validation strategies including: Imaging (Core in Neurobiology), ELISAs (TPC,TEID), and Kinetic assays (TPC, TEID).
- Provide workshops and trainings through the INBRE program and Increase users and visits to the TPC, as well as supported projects.
Yadira M. Cantres, BS