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Central Electron Microscopy Unit

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Description of Facilities 

The Central Electron Microscope Unit (CEMU) is a shared advanced instrumentation laboratory of the Medical Sciences Campus. Developed under the aegis of the RCMI Program, this Unit maintains and operates electron microscope facilities for the faculty, investigators and students of the Medical Sciences Campus for research, training and diagnosis. 

The Unit, located in the basement of the main Medical Sciences Campus building, occupies an area of approximately 2,000 square feet and consistsof two specimen preparation laboratories, four electron microscope suites, a fully equipped darkroom, a microtomy room, and two offices. One of the processing laboratories contains all the ancillary instruments and equipment required to process samples for transmission electron microscopy, the other for scanning electron microscopy. The microtomy laboratory contains six cubicles, each of which houses an ultramicrotome; some of the cubicles are also equipped with a compound microscope for examination of thick sections. A Nikon compound microscope with camera attachment is available for microphotography,and a Bowens Illumitrans copier for preparation and duplication of slides. 

For more information please see the Unit Guidelines


Major Instrumentation 

  • JEOL 1200-EX STEM with EDAX unit for microanalysis and digital-image processor 
  • 2 Philips 201 transmission electron microscopes 
  • ETEC scanning electron microscope 
  • 3 LKB ultrotomes with supporting tables 
  • 2 Huxley microtomes 
  • JB-4 microtome 
  • Biorad E5200 Auto Sputter coater 
  • Biorad CPD 750 Critical Point Drier 
  • Beckman Microfuge E 
  • Acumet pH meter 
  • Osmometer 
  • Branson 2200 ultrasonic cleaner 
  • Bowens Illumitrans slide copier 
  • 3 Nikon compound microscopes 
  • American Optical dissecting microscope 
  • Mettler AE 240 analytical balance 
  • Arkay RC-1100 print dryer 
  • Nikon F3 camera with a macro lens 
  • 4 Dehumidifiers 
  • Oven 
  • Ice maker 
  • Refrigerator 
  • Freezer 
  • Light table 
  • Nikon Optiphot Microscope
Services Provided 
  • Light microscopy 
  • Transmission electron microscopy 
  • Scanning Electron Microscopy 
  • Immunogold labelling 
  • Microtomy 
  • Osmometry 
  • Photographic processing 
  • Technical Support 
  • Technical Training 
  • Seminars and Workshops 

Technical Staff
  • Eng. Camillo Cangani Cristofori
    UPR-Medical Sciences Campus
    Tel. 758-2525 x1004; x1018
    Email: ccangani@rcm.upr.edu
  • Radamés Rosso
    Clinical Diagnostics Supervisor 

Activity Coordinator

Wieslaw Kozek, Ph.D.
Director
Email: wkozek@rcm.upr.edu
Telephone: (787) 758-2525
Ext. 1004, 1018, 1351

Location 
Rooms BB-11A-E, BB-08A-B, BB-07A-C 
Basement, Main Building, 
Medical Sciences Campus 

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The project is supported by Grant Number G12RR03051(RCMI Program, University of Puerto Rico Medical Sciences Campus) from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH.