Dr. Michael Brad Strader, a researcher from National Institutes of Health (NIH) visits the University of Puerto Rico Medical Sciences Campus
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| What | Seminar |
|---|---|
| When |
Monday, September 27, 2004 from 09:00 to 12:00 |
| Where | Room A-371, 3rd FloorUPR-Medical Sciences Campus, Main Building |
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RCMI Seminars Series
WORKSHOP: Current Concepts and Techniques for Protein Biomarker Discovery and Identification
James E. Evans
Director of the Proteomics and Mass Spectrometry Facility
University of Massachusetts Medical School
James A. Mobley
Research Instructor
Vanderbilt University
Dr. Irene Bosch
Assistant Professor
Center for Infectious Disease and Vaccine Research
University of Massachusetts Medical School
Lecture 1: Biomarker Discovery via Mass Profiling – the Importance of Quality Mass Spectrometry and Data Analysis.
Topics covered:
- SELDI vs. MALDI - When to apply each and why.
- The pros, cons, and current techniques in profiling mass spectrometry - review of the literature.
- Assessing and applying instrumental, experimental, and population error prior to data analysis.
- Biomarker analysis - weeding out those important proteins in-silico.
- The importance of maintaining a Top Down approach in protein ID.
Lecture 2: Protein Identification by Mass Spectrometry.
Topics covered:
- Mass Spectrometry Instrumentation for Proteomics – the Basics.
- Protein Identification by MALDI Mass Mapping.
- Protein Identification by nano-LC-MS/MS.
- Techniques for Identification and Quantification of Proteins in Mixtures by nano-LC-MS/MS.
Sponsored by: the RCMI AIDS and Emerging Infectious Diseases Activity, and Dept. of Microbiology
