Description of Facilities 

The Image Analysis Resource Center was established in 1996. A previous instrumentation grant from the Department of Defense in 1993 provided the basic equipment. From 1996-2001 the RCMI Program provided funds to purchase additional equipment and technical support to train investigators in the use of Image Analysis and to supervise the facilities. The objectives of the Center for 2001-2006 are to add additional instrumentation (i.e. confocal microscope) and capabilities (i.e. introducing investigators to "NIH Image", a free imaging software) as well as to make the facility available to a larger community of investigators.

Current research projects utilizing the facilities include:

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• Prenatal Stress, Opioids, and Cocaine Sensitization  (PDF - 119Kb)
  Annabell Segarra, Ph.D.
  
  
• HIV Sensory Neuropathy: Age Risk Factors and Immunopathogenesis
  Carlos Luciano, M.D.
  
  
• Double Transfectant of hP2Y2 Receptor and Caveolin-1 in 1321N1 Astrocytoma Cells (AVI - 2.8Mb)
  Edna Aquino, Ph.D., Walter Silva, Ph.D. and Dr. Fernando González, Ph.D.
  
  
• Expression of Insulin Receptor-EGFP fusion protein in stable transfected Molt-4 T cells (AVI - 2.3Mb)
  Emma Fernández-Repollet, Ph.D.
  
  
• Expression of EphA7 after spinal cord trauma (AVI - 1.2Mb)
  Jorge Miranda, Ph.D.
  
  
• Role of EphA Receptors and Eprins Ligands After Spinal Cord Injury
  Jorge Miranda, Ph.D.
  
  
• Structural, Biophysical, and Behavioral Consequences of Allosteric Modulation of GABAA-Receptors by Neurosteriods  (PDF - 62Kb)
  Juan Carlos Jorge, Ph.D.
  
  
• Cell Comunication in the Diabetic Heart
  Walmor DeMello, Ph.D.
  
  

• Morphometry - area, volume, perimeter, length, width, etc. 
• Cell counting 
• 3-D reconstruction 
• Grain counting for in situ hybridization 
• Densitometry - gel analysis, autoradiography 
• Bone histomorphometry 
• Real time image analysis - calcium measurements 

• HIV Sensory Neuropathy: Age Risk Factors and Immunopathogenesis. 
• Estrogen regulation of cocaine-induced locomotor sensitization. 
• Cell comunication in the diabetic heart. 
• Structural, biophysical, and behavioral consequences of allosteric modulation of GABAa receptors by neurosteroids. 
• Role of Epha receptors and eprins ligands after spinal cord injury. 
• Determination of the relative expression of caveolin isoforms during the differentiation of C6 astroglial cells. 
• Involvement of Myoip in the movement of Chs3p and Sncip inside Saccharomyces cereviciae cells.